|
Document Type |
: |
Thesis |
|
Document Title |
: |
Biodegradation of petroleum hydrocarbons by thermophilic bacterial consortia isolated from oil drilling site in eastern region of Saudi Arabia التحلل الحيوي لهيدروكربونات البترول الخام بواسطة مجموعة من البكتريا المقاومة للحرارة والمعزولة من موقع لتنقيب النفط في المنطقة الشرقية بالمملكة العربية السعودية |
|
Subject |
: |
Faculty of Sciences > biological sciences department |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
A thermophilic oil-degrading bacterial consortium consisting of two isolates HD1 and HD2 was isolated from oil drilling site at 8000 feet from Saudi Aramco Company, Alkobar, Saudi Arabia. Biodegradation of low molecular weight of polycyclic aromatic hydrocarbons (PAHs) like phenanthrene, fluorene, anthracene, and naphthalene were studied at 50C as a sole carbon source on mineral salt medium (MSM) at different concentrations (25, 50, 100, 200, 500, 1000 and 1500) ppm. Bacterial growth was detected using viable cell count and protein estimation. The results revealed that thermophilic bacterial consortium degraded (95) % of naphthalene, (90) % of fluorene, (85) % of phenanthrene and (80) % of anthracene detected by high performance liquid chromatography (HPLC). PAHs mineralization study was carried out using respirometer. Gas chromatography mass spectrometer (GCMS) was used to identify the metabolites formed during the PAH degradation. Lab. scale bioreactor was performed with petroleum wastewater collected form the pipeline near the refinery, Yanbu, Saudi Arabia. The bioreactor study showed that 96% of the organic compounds were removed from the petroleum wastewater within 24 days. The isolated consortium consisting of two isolates HD1, and HD2 was characterizes and identified by morphological and biochemical tests as Bacillus sp. and Pseudomonas sp. Identification was confirmed by 16S rRNA sequencing using basic local alignment search tool for nucleotides (BLASTN) which showed that HD1 was 69% homology to Bacillus butanolivorans, and HD2 was 99% homology to Pseudomonas alcaligenes. |
|
Supervisor |
: |
Prof. Dr. Magda Mohammad Aly |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1437 AH
2016 AD |
|
Co-Supervisor |
: |
Dr. Huda Abdulatief Qari |
|
Added Date |
: |
Wednesday, June 29, 2016 |
|
Researchers
| هديل عباد وازن | Wazin, Hadeel Abbad | Researcher | Master | |
|