Document Details

Document Type : Thesis 
Document Title :
Chromatographic Studies on the Chemical Constituents of Hair Dyes
دراسات كروماتوجرافية على التركيب الكيميائي لصبغات الشعر
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Hair dyes are now commonly used in Saudi Arabia and other nations across the world to cover up grey hairs or modify the natural color of the hair. The aromatic amines and phenols which are the principal ingredients of hair dyes may cause allergic reactions, irritated skin, and even cancer in certain cases. The aim of this study is to separate and identify the four chemical constituents present in the commercial hair dyes that are available in the local market. High Perfomance Liquid Chromatographic technique was employed to identify the toxic chemical compounds that are present in twenty six hair dyes samples representing ten famous brands. Comparison between many shades of dyes significantly revealed the hazards of these dangerous chemical constituents. Four chemical substances are separated and identified namely: P-phenylenediamine, m-aminophenol, resorcinol, and 2-methylresorcinol in commercial oxidative hair dyes using a simple and reliable RP-HPLC technique. Different interaction reagents were used to enhance the separation of amines which were added to the mobile phase. This mobile phase was (10:90, vol/vol.) methanol-aqueous solution containing 0.05% diethanolamine and 0.02 M of ammonium acetate at pH 5.2. The column was 150 x 4.6 mm ID and 5µm Eclipse Plus C18 column. The UV detector was set at two different wavelengths 245 and 233nm. The retention time were 1.0, 2.2, 3.7, and 4.5 minutes for P-phenylenediamine, m-aminophenol, resorcinol, and 2-methylresorcinol, respectively. The calibration curves for all four standards demonstrate excellent linearity and coefficient of determination (R2 > 0.9902) which were achieved in a different range of concentrations. The results confirmed the effectiveness of the method proposed to separate and identify the four hair dye constituents in terms of high sensitivity and selectivity and excellent limit of detection and quantification. 
Supervisor : Prof. Dr. Saleh Omar S. Bahaffi 
Thesis Type : Master Thesis 
Publishing Year : 1444 AH
2023 AD 
Co-Supervisor : Dr. Taghreed Mohammed I. Fagieh 
Added Date : Friday, May 5, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
خلود عبدالعزيز الزهرانيAlzahrani, Khulood AbdulazizResearcherMaster 

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 49187.pdf pdf 

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