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Document Type |
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Thesis |
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Document Title |
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Biochemical and histological studies on the effect of the Patulin mycotoxin on male rats’ liver and the use of crude venom extracted from jelly fish as a treatment دراسات كيميائية حيوية ونسيجية على تأثير السم الفطري باتولين على كبد ذكورالجرذان واستخدام السم الخام المستخلص من قنديل البحر كعلاج. |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Mycotoxins contaminate a quarter of the world food crops, including many basic foodstuffs and animal feed with high economic value. Patulin is considered to be the most important mycotoxin in fruit juices, and is produced by several fungal species including Penicillium, Aspergillus and Byssochlymus of which Penicillium expansum causes the most common storage rot of apples. It mainly found on fruit particularly apples and apple products and can be found on a variety of other moldy fruits, vegetables, stored cheese and cereal Products. The major potential dietary sources of Patulin are apples and apple juices made from affected fruit. The current work presented in this study deals with the effect of Patulin mycotoxin on some biochemical parameters and histological changes on male rats’ liver and also, the effect of the crude venom extracted from jelly fish Cassiopea Andromeda as a treatment. To investigate these effects, 50 inbreeding weanling white male wistar lewis rats were divided randomly into 5 groups (10 rats each). Control group was gavage fed daily with distilled water; three other treated groups were gavage fed daily dose with Patulin (0.2 mg/kg b. w.) for one, two and three weeks respectively. The last fifth group was treated by Patulin as treated one for one week then injected intraperitoneally with a single dose of crude venom from jelly fish (1.78 mg/ 20 g b.w.) for 24 hours according to LD50 .
Serum enzymes activity is often used clinically to assess liver diseases such as transaminases and gamma glutamyl transferase (GGT). The level of asparatate aminotransferase (AST) and (GGT) were increased significantly in serum of all treated groups compared with control group but the level of alanine aminotransferase (ALT) was increased significantly in treated group after one week only. Although the concentration of tumor necrosis factor alpha (TNF-α) was increased significantly and gradually in all treated groups, the concentration of ferritin was decreased significantly in treated three after three weeks only. Also, serum glucose was significantly increased in treated groups after two and three weeks. The glycogen concentration in liver homogenate was decreased in treated groups but not significant comparing to control group. The recovery occurred in treated four that was treated by single dose of crude venom after Patulin administration based on improvement the level of (AST, ALT, GGT and TNF-α) when comparing with treated one which treated by Patulin only. The toxins of coelenterates had been detected to include bradykinnin and related polypeptides which in turn improved hepatic function. The other parameters were not realized significant change which parallel to the results of treated one when compared with control group. Histopathological changes of rat liver coincided with biochemical changes. In conclusion, the oral exposure to Patulin indicated that hepatic alteration was produced in a manner related to dose duration. The crude venom from jelly fish might be used as new therapeutic approach to enhance hepatic detoxification of Patulin mycotoxin. |
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Supervisor |
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Prof. Nagwa M. El- Sawi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1431 AH
2010 AD |
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Co-Supervisor |
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د. هناء محمد قشلان |
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Added Date |
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Saturday, July 3, 2010 |
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Researchers
| رحاب فضل المصعبي | Al-Massabi, Rehab Fadhl | Researcher | Master | |
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